1
0
Fork 0
mirror of https://github.com/tldr-pages/tldr.git synced 2025-04-22 06:22:07 +02:00

bwa: add page (#12720)

* bwa: add page

---------

Co-authored-by: CleanMachine1 <78213164+CleanMachine1@users.noreply.github.com>
Co-authored-by: K.B.Dharun Krishna <kbdharunkrishna@gmail.com>
Co-authored-by: Sebastiaan Speck <12570668+sebastiaanspeck@users.noreply.github.com>
This commit is contained in:
Maciej Michalczyk 2024-05-14 15:57:17 +02:00 committed by GitHub
parent 7c4ebf35b6
commit b6ea0b654c
No known key found for this signature in database
GPG key ID: B5690EEEBB952194

25
pages/linux/bwa.md Normal file
View file

@ -0,0 +1,25 @@
# bwa
> Burrows-Wheeler Alignment tool.
> Short, low-divergent DNA sequences mapper against a large reference genome, such as the human genome.
> More information: <https://github.com/lh3/bwa>.
- Index the reference genome:
`bwa index {{path/to/reference.fa}}`
- Map single-end reads (sequences) to indexed genome using 32 [t]hreads and compress the result to save space:
`bwa mem -t 32 {{path/to/reference.fa}} {{path/to/read_single_end.fq.gz}} | gzip > {{path/to/alignment_single_end.sam.gz}}`
- Map pair-end reads (sequences) to the indexed genome using 32 [t]hreads and compress the result to save space:
`bwa mem -t 32 {{path/to/reference.fa}} {{path/to/read_pair_end_1.fq.gz}} {{path/to/read_pair_end_2.fq.gz}} | gzip > {{path/to/alignment_pair_end.sam.gz}}`
- Map pair-end reads (sequences) to the indexed genome using 32 [t]hreads with [M]arking shorter split hits as secondary for output SAM file compatibility in Picard software and compress the result:
`bwa mem -M -t 32 {{path/to/reference.fa}} {{path/to/read_pair_end_1.fq.gz}} {{path/to/read_pair_end_2.fq.gz}} | gzip > {{path/to/alignment_pair_end.sam.gz}}`
- Map pair-end reads (sequences) to indexed genome using 32 [t]hreads with FASTA/Q [C]omments (e.g. BC:Z:CGTAC) appending to a compressed result:
`bwa mem -C -t 32 {{path/to/reference.fa}} {{path/to/read_pair_end_1.fq.gz}} {{path/to/read_pair_end_2.fq.gz}} | gzip > {{path/to/alignment_pair_end.sam.gz}}`